When Is The Best Time To Test For Hormone Imbalance?

Have you noticed pimples on your skin, or are your periods irregular? If yes, you are most likely to have a hormone imbalance. Teenagers or older women are more susceptible to hormone imbalance than other age groups. Moreover, it can affect your mental health and disturb your mood. In such a scenario, you might be wondering when is the best time to test for hormone imbalance.

Keep reading to find out when is the best time to test for hormone imbalance.

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What is a Hormone Profile Test?

Your endocrine system is mainly responsible for producing and releasing over 50 hormones. A hormone profile test is a blood test that helps to find the day-by-day changes occurring in the hormonal levels. This test also assists your healthcare provider in establishing a correct diagnosis of diabetes, thyroid diseases, or fertility disorders.

If you are expecting changes in the following hormones, this test will aid you to rule out your condition:

  • Progesterone
  • Estrogen
  • Luteinizing hormone
  • Follicle-stimulating hormone
  • Testosterone
  • DHEA-S
  • Thyroid hormones (T3, T4, TSH)
  • Insulin
  • Cortisol

When Should I Get A Hormone Profile Test?

If you’re experiencing the following signs and symptoms, you’re at higher risk of developing hormonal imbalance, and therefore, you get your hormone profile test done.

  • Irregular or painful menstruation 
  • Disturbed bowel movements
  • Loss or gain of appetite 
  • Unexplained weight loss or weight gain
  • Extreme sweating
  • Frequent muscle spasms, stiffness, or weakness
  • Inflamed or swollen joints
  • Inconsistent heart rate
  • Fatigue
  • Skin rashes or dryness
  • Thinning of the hair
  • Sensitivity to hot or cold temperature 
  • Inability to concentrate 
  • Incidence of depression, anxiety, or irritability 

Why Does Timing Of The Hormone Profile Test Matter?

Certain factors influence the release and productivity of hormones. Time also plays a vital role in activating and inhibiting various hormone secretions. 

For instance, your endocrine system releases melatonin hormones in response to darkness that help modulate your sleep cycles. Similarly, hormones such as cortisol and serotonin are at their highest concentration during daylight. 

That’s why measuring the hormonal levels at inappropriate times can give false or misleading results. 

Following are some of the reasons that can impact the results of hormone profile tests:

Female Menstrual Cycle

If you’re a teenage girl or adult woman, you tend to experience menstruation every month. It is a regular process controlled by the release of certain hormones.

Moreover, your hormone levels fluctuate depending on the phase of your ongoing menstrual cycle. You’re most likely to undergo these hormonal changes during your menstrual cycle:

  • Follicular Phase – Follicular phase starts from day 1 of your menstrual cycle and ends on day 14. During this phase, your body tends to go through the process of maturing an egg. Both follicle-stimulating hormone and estrogen are at the highest concentration in the follicular phase.
  • Luteal Phase – Luteal phase starts from day 15 of your menstrual cycle and ends on 28 days. During this phase, your body prepares to release an egg (ovum) through ovulation. Progesterone hormones tend to increase in this phase, whereas the estrogen level goes down.


In addition, when women undergo the cessation of menstruation, also known as menopause, they are at greater risk of developing hormonal imbalances. You are most likely to experience lower levels of progesterone and estrogen after getting menopause. 


As you age, your body becomes more susceptible to wear and tear. Similarly, your endocrine system gets affected by aging, which results in an imbalance of hormones, especially sex hormones. 

Aging influences the release of these hormones, resulting in their lower concentrations:

  • Testosterone Hormone
  • Estrogen Hormone
  • Progesterone Hormone
  • Aldosterone Hormone 
  • Cortisol Hormone
  • DHEA-S Hormone
  • Thyroid Hormone

However, parathyroid hormone concentration gets higher with increasing age. It helps in releasing the calcium from your bones. This is why you experience low bone mineral density in older ages. 

Time of the Day

Your hormone concentration does vary in the morning and night. Certain hormone levels go up or down depending on the daylight; this process is called diurnal variations. Hormones that control stress and sleep cycle are more likely to be influenced by diurnal variations. Moreover, when is the best time to test for hormone imbalance changes due to diurnal variations?

These hormones include:

  • Melatonin 
  • Cortisol
  • Serotonin 
  • Prolactin 
  • Testosterone in men
  • Growth Hormone (GH)
  • Thyroid-stimulating hormone (TSH)

Other Factors That Influence The Results Of Hormone Profile Test

Other factors that can influence when is the best time to test for hormone imbalance are:

  • Medications
  • Illness or infections 
  • Pregnancy
  • Hormonal birth control
  • Eating habits 
  • Physical training or exercise
  • Sleeping patterns
  • Stress levels

These factors can affect the accuracy of your hormone profile test and may give false or misleading results. 

When is the Best Time to Test Female Hormones?

The female body consists of hormones such as estrogen, progesterone, and testosterone that help maintain the menstrual cycle.

The best time to test female hormones is:


The best time to test estrogen levels lies between days 19-22 of your menstrual cycle. Your body may undergo heavy periods, irritability, and weight gain due to elevated estrogen levels during the luteal phase. 


Progesterone levels upsurge during the 5 to 7 days of ovulation during the menstrual cycle’s luteal phase. That’s why the recommended time to check progesterone level is days 19-22 of the female reproductive cycle to get accurate results.


Testosterone is a male hormone, but it is present in female bodies in lesser quantities. When it prevails in high concentration in females, it causes polycystic ovarian syndrome (PCOS), acne, irregular periods, and hair growth on the chin, chest, and abdomen. You can check testosterone levels any time of the month, but it’s better to evaluate it in the morning when its concentration is at its highest. 

Where To Get Effective and Natural Hormone Restoration Therapy in NYC, USA?

At MOCEAN Wellness Center in NYC, our experts identify the cause of hormonal imbalance before suggesting any therapy. We are well-equipped with modern wellness technologies such as sound room, red light therapy, and salt room to balance your hormones naturally.

Also, our professionals will guide you when is the best time to test for hormone imbalance to get accurate results. 

The Bottom Line - When is the Best Time to Test For Hormone Imbalance

Your endocrine system produces and releases numerous hormones to regulate your body’s functions. However, these functions get highly affected when you possess hormonal imbalances. That’s why if you experience irregular periods, extreme lethargy, or mood swings, you should seek medical advice and undergo a hormone profile test. 

Book an appointment at our longevity center in NYC to restore your hormonal imbalance naturally by calling us right now! 

Then we create a health plan tailored to your needs.

Based on the results, we develop a personalized treatment plan for each individual to promote optimal health. With 1-on-1 consultations, cutting-edge technology, and a team of experts who care about getting you better, MOCEAN offers you a comprehensive road map on how to become the healthiest version of yourself.